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目的探索建立积雪苷滴丸的鉴别和含量测定的方法。方法TLC法鉴别积雪苷滴丸中积雪草苷和羟基积雪草苷,HPLC法测定积雪草苷的含量。结果TLC法鉴别积雪草苷和羟基积雪草苷结果满意,HPLC法测定积雪草苷的含量,线性范围:y=0.234x+49.869,r=0.9999,检出线性范围:0.91~45μg,加样回收率为98.9%。结论上述方法准确、专属性强,可作积雪苷滴丸的定性和定量分析方法。
Objective To explore the method for identification and determination of asiaticoside dropping pills. Methods TLC method was used to identify asiaticoside and madecassoside in asiaticoside dropping pills. HPLC method was used to determine asiaticoside content. Results The results of TLC method for the identification of Asiaticoside and madecassoside were satisfactory. The HPLC method was used to determine the content of asiaticoside. The linear range was y=0.234x+49.869, r=0.9999, and the detection linear range was 0.91~45μg. The recovery rate was 98.9%. Conclusion The above method is accurate and specific. It can be used as a qualitative and quantitative analysis method for asiaticoside dropping pills.