论文部分内容阅读
为了研究CGRPmRNA、PPTAmRNA和NOS在伤害性刺激后大鼠TG中合成和表达的变化,将4%鹿角菜胶(CAR)溶液注射到大鼠一侧口周皮下作为伤害性刺激模型,用NADPH-d组织化学法结合CGRP、PPTAmRNAs原位杂交组织化学法对此进行了观察。结果显示:对照组大鼠TG中约有32.2%、16.2%和10%的神经元分别呈CGRPmRNA、PPTAmRNA和NOS阳性。CAR刺激后,在刺激侧TG、CGRPmRNA、PPTAmRNA和NOS阳性神经元的数量均增多,其阳性率分别达到了38.6%、22.8%和14.2%。TG中约有6.8%和7.3%的神经元同时呈NOS和CGRPmRNA或NOS和PPTAmRNA阳性,CAR刺激使得两种双标神经元的数量也明显增多,双标细胞占TG细胞总数的百分比分别达到了11.0%和10.9%,而且刺激后增加的NOS阳性神经元主要为双标神经元。上述结果提示CGRPmRNA、PPTAmRNA和NOS阳性神经元,尤其是共存神经元可能在面口部伤害性信息的传递和调节中起重要作用,伤害性刺激所诱导的NOS合成的增加与CGRPmRNA和PPTAmRNA表达的增强可能有密切的关系。
To investigate the changes of synthesis and expression of CGRP mRNA, PPTA mRNA and NOS in rat TG after noxious stimulation, 4% carrageenan (CAR) solution was injected into the peri-subcutaneous peritoneal space of rats as nociceptive stimulus model, d histochemical method combined with CGRP, PPTAmRNAs in situ hybridization histochemical method was observed. The results showed that about 32.2% of rats in control group had TG, and 16.2% and 10% of neurons were positive for CGRP mRNA, PPTA mRNA and NOS, respectively. After stimulation with CAR, the numbers of TG, CGRP mRNA, PPTAmRNA and NOS positive neurons increased on stimulation side, and the positive rates were 38.6%, 22.8% and 14.2% respectively. About 6.8% of TG and 7.3% of neurons were positive for both NOS and CGRP mRNA or NOS and PPTAmRNA. The number of two double-labeled neurons was also significantly increased by CAR stimulation. The number of double-labeled cells in total TG cells Percentages reached 11.0% and 10.9% respectively, and the increased NOS-positive neurons after stimulation were mainly double-labeled neurons. The above results suggest that CGRP mRNA, PPTA mRNA and NOS positive neurons, especially coexisting neurons, may play an important role in the transmission and regulation of noxious nociceptive information. The increase of NOS synthesis induced by noxious stimulation is related to the increase of CGRP mRNA and PPTA mRNA expression Enhancement may be closely related.