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探索有机磷酸酯类化合物对大鼠γ 氨基丁酸受体的体外影响 ,为有机磷酸酯类化合物急性中毒的体内研究提供理论依据。用大鼠脑神经末梢微囊GABA受体为模型 ,观察二嗪农、梭曼、益棉磷、乙硫磷、杀螟松、丙氟磷等有机磷酸酯类化合物对大鼠GABA受体的体外反应变化。结果观察到10mmol/L益棉磷、杀螟松、丙氟磷或 5mmol/L二嗪农、梭曼均明显降低大鼠GABA受体与3 H GABA的结合率 (P <0 0 1) ,0 1mmol/L梭曼或 0 5mmol/L二嗪农仍明显降低GABA受体与3 H GABA的结合率 (P <0 0 5 )。表明有机磷酸酯类化合物在一定浓度下体外反应能显著降低大鼠脑GABA受体与3 H GABA的结合率 ,GABA受体参与了某些有机磷酸酯类化合物急性中毒过程。
To explore the in vitro effects of organophosphate compounds on γ-aminobutyric acid receptor in rats, and to provide a theoretical basis for the in vivo study of organophosphate compounds acute poisoning. The GABA receptor of rat brain nerve endings microcapsules was used as a model to observe the effects of diazinon, soman, YPC, etoposide, fenitrothion and isoflurane on GABA receptor In vitro response changes. Results The binding rate of GABA receptor to 3 H GABA in rats was significantly decreased (P <0.01) with or without 10 mmol / L oxalicum, fenitrothion, propafenone or 5 mmol / L diazinon and soman, 0 1 mmol / L soman or 0 5 mmol / L diazinon still significantly reduced the binding rate of GABA receptor to 3 H GABA (P <0 05). The results showed that the organophosphate compounds could significantly reduce the binding of GABA receptor to 3 H GABA in rat brain at a certain concentration. GABA receptor was involved in the acute poisoning of some organophosphates.