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根据Osborne关于小麦蛋白质组分的定义,采用连续振荡提取分离法提取小麦种子中的清蛋白、球蛋白、醇溶蛋白和谷蛋白,并用凯氏定氮法对提取物进行定量,通过结果分析选择出提取分离蛋白质组分的顺序和提取时间为;清蛋白振荡离心提取5次,提取总时间为110min;球蛋白振荡离心提取5次,提取总时间为110min;醇溶蛋白振荡离心提取3次,提取总时间为70min;谷蛋白振荡离心提取3次,提取总时间为110min。
According to the Osborne definition of wheat protein components, the extraction of albumin, globulin, prolamin and glutelin in wheat seeds was performed by continuous oscillating extraction and separation, and the extracts were quantified by Kjeldahl method. The extraction and separation of the protein components of the order and extraction time; centrifugation of the albumin was centrifuged five times, the total extraction time was 110min; centrifugation globulin globulin 5 times, the total extraction time was 110min; ginkgoins centrifuged three times, The total extraction time was 70min. The gluten was centrifuged for 3 times and the total extraction time was 110min.