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为提高抗原表达质粒在重组伤寒沙门氏菌中的稳定性以增强重组伤寒沙门氏菌诱导的免疫应答 ,克隆鼠伤寒沙门氏菌pagC基因启动子 ,以其为转录调控元件构建HCV核心抗原表达质粒 ,转化到减毒鼠伤寒沙门氏菌中。体外培养时 ,Mg2 +能够剂量依赖性抑制该重组菌表达HCV核心抗原。将该重组菌和组成性表达的重组菌分别口服接种BALB/c小鼠 ,观察质粒的稳定性和小鼠的免疫应答。结果表明 ,体内激活的pagC基因启动子能明显提高质粒在重组鼠伤寒沙门氏菌中的稳定性和增强重组菌诱导的体液和细胞免疫应答 ,这为发展高效免疫、成本低廉的口服丙肝疫苗提供了一个新思路
In order to improve the stability of antigen expression plasmid in recombinant Salmonella typhimurium and enhance the immune response induced by Salmonella typhimurium, the promoter of pagC gene of Salmonella typhimurium was cloned and used as transcriptional regulatory element to construct HCV core antigen expression plasmid and transformed into attenuated rat Salmonella typhi. When cultured in vitro, Mg2 + could inhibit the expression of HCV core antigen in a dose-dependent manner. BALB / c mice were orally inoculated with the recombinant bacteria and the constitutively expressed recombinant bacteria respectively to observe the stability of the plasmid and the immune response of the mice. The results show that in vivo activation of the pagC gene promoter can significantly improve the stability of the recombinant plasmid in Salmonella typhimurium and enhance the humoral and cellular immune responses induced by recombinant bacteria, which provides an effective and cost-effective oral hepatitis C vaccine New ideas