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目的:建立用HPLC法测定盆炎净胶囊中绿原酸含量的方法。方法:色谱柱:XBridge~(TM)-C_(18)(150 mm×4.6 mm,5μm),流动相:甲醇-0.1%磷酸溶液(15:85),流速:1.0 ml·min~(-1),检测波长:327 nm。结果:绿原酸线性范围:0.03～0.46μg,回归方程:Y=2.58×10~6X-2.07×10~4(r=0.999 8),加样回收率:97.89%,RSD=1.31%(n=6)。结论:本法操作简便,准确,重复性好,为测定盆炎净胶囊中绿原酸的含量提供了准确可靠的分析方法。
Objective: To establish a method for the determination of chlorogenic acid in Penyin Capsule by HPLC. METHODS: The mobile phase consisted of XBridge ~ (TM) -C 18 (150 mm × 4.6 mm, 5 μm), mobile phase methanol-0.1% phosphoric acid solution (15:85), flow rate of 1.0 ml · min -1 ), Detection wavelength: 327 nm. Results: The linear range of chlorogenic acid was 0.03-0.46μg. The regression equation was: Y = 2.58 × 10-6X-2.07 × 10-4 (r = 0.999 8). The recoveries were 97.89%, RSD = 1.31% = 6). Conclusion: The method is simple, accurate and reproducible. It provides an accurate and reliable method for the determination of chlorogenic acid in Penyanjing Capsule.