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目的探讨人巨细胞病毒（ＨＣＭＶ）对人骨髓粒巨噬系祖细胞集落（ＣＦＵＧＭ）是否有直接抑制作用。方法采用造血祖细胞体外培养技术，研究病毒株ＨＣＭＶＡＤ１６９对ＣＦＵＧＭ生长的影响，用原位聚合酶链反应（ＩＳＰＣＲ）技术检测ＣＦＵＧＭ的细胞内ＨＣＭＶＡＤ１６９ＤＮＡ。结果高浓度ＨＣＭＶＡＤ１６９（２×１０６ｐｆｕ／ｍｌ和２×１０５ｐｆｕ／ｍｌ）在体外能明显抑制ＣＦＵＧＭ的生长（Ｐ＜０．０１），而低浓度（２×１０４ｐｆｕ／ｍｌ）ＨＣＭＶＡＤ１６９则无此作用，ＨＣＭＶＡＤ１６９对骨髓ＣＦＵＧＭ的抑制作用因其浓度的不同而有差异；经ＩＳＰＣＲ检测发现在ＣＦＵＧＭ的细胞核中存在ＨＣＭＶＡＤ１６９ＤＮＡ。结论ＣＦＵＧＭ是ＨＣＭＶＡＤ１６９的宿主细胞之一，ＨＣＭＶＡＤ１６９对骨髓ＣＦＵＧＭ生长的抑制作用与其对ＣＦＵＧＭ的直接感染有关。
Objective To investigate whether human cytomegalovirus (HCMV) has a direct inhibitory effect on CFU-GM in human bone marrow-macrophage-derived progenitor cells. Methods The hematopoietic progenitor cells cultured in vitro techniques were used to study the effect of HCMVAD169 on the growth of CFUMG. The intracellular HCMVAD169DNA of CFUGM was detected by in situ polymerase chain reaction (ISPCR). Results High concentrations of HCMVAD169 (2 × 106pfu / ml and 2 × 105pfu / ml) could significantly inhibit the growth of CFUGM in vitro (P <0.01), while the low concentration (2 × 104pfu / ml) of HCMVAD169 There is no such role, HCMV AD169 on bone marrow CFU MG inhibition due to its concentration of different differences; by ISPCR test found in the nucleus CFM GM CGMAD AD169 DNA exists. Conclusions CFU-GM is one of the host cells of HCMV-AD169. The inhibitory effect of HCMV-AD169 on the growth of bone marrow CFU-MG is related to the direct infection of CFU-GM.