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Aim:To investigate the effects of sphingosylphosphorylcholine(SPC)on humanmonocyte-derived dendritic cell(DC)chemotaxis.Methods:Human DC were gen-erated from peripheral blood monocytes by culturing them with granulocyte mac-rophage-colony stimulating factor and interleukin-4.The effect of SPC on the DCchemotactic migration was measured by chemotaxis assay.Intracellular signalingevent involved in the SPC-induced DC chemotaxis was investigated with severalinhibitors for specific kinase.The expression of the SPC receptors was examinedby reverse transcription polymerase chain reaction.Results:We found that SPCinduced chemotactic migration in immature DC(iDC)and mature DC(mDC).Interms of SPC-induced signaling events,mitogen activated protein kinase activa-tion and Akt activation in iDC and mDC were stimulated.SPC-induced chemotaxiswas mediated by extracellular signal-regulated protein kinase and phosphoino-sitide-3-kinase,but not by calcium in both iDC and mDC.Although mDC expressovarian cancer G protein-coupled receptor 1,but not G protein-coupled receptor 4,iDC do not express any of these receptors.To examine the involvement of sphin-gosine-1-phosphate(S1P)receptors,we checked the effect of an S1P receptorantagonist(VPC23019)on SPC-induced DC chemotaxis.VPC23019 did not affectSPC-induced DC chemotaxis.Conclusion:The results suggest that SPC may playa role in regulating DC trafficking during phagocytosis and the T cell-stimulatingphase,and the unique SPC receptor,which is different from SIP receptors,isinvolved in SPC-induced chemotaxis.
Aim: To investigate the effects of sphingosylphosphorylcholine (SPC) on humanmonocyte-derived dendritic cell (DC) chemotaxis. Methods: Human DC were gen-erated from peripheral blood monocytes by culturing them with granulocyte mac-rophage-colony stimulating factor and interleukin-4 The effect of SPC on the DC actotype was measured by chemotaxis assay. Intracellular signal in gene involved in the SPC-induced DC chemotaxis was investigated with several inhibitors for specific kinase. The expression of the SPC receptors was examined by reverse transcription polymerase chain reaction. Results: We found that SPCinduced chemotactic migration in immature DC (iDC) and mature DC (mDC). Interms of SPC-induced signaling events, mitogen activated protein kinase activa tion and Akt activation in iDC and mDC were stimulated. SPC-induced chemotaxis was mediated by extracellular signal-regulated protein kinase and phosphoino-sitide-3-kinase, but not by calcium in both iDC and mDC.Although mDC expressovarian ca ncer G protein-coupled receptor 1, but not G protein-coupled receptor 4, iDC do not express any of these receptors. To examine the involvement of sphin-gosine-1-phosphate (S1P) receptors, receptorantagonist (VPC23019) on SPC-induced DC chemotaxis. VPC23019 did not affect SPC-induced DC chemotaxis. Conlusion: The results suggest that SPC may play role in regulating DC trafficking during phagocytosis and the T cell-stimulating phase, and the unique SPC receptor, which is different from SIP receptors, isinvolved in SPC-induced chemotaxis.