论文部分内容阅读
目的探讨雷公藤单体T4对胰岛β细胞的免疫保护作用,为胰岛β细胞的免疫保护治疗提供实验依据。方法培养胰岛β细胞株NIT细胞及正常对照外周血单个核细胞,分别与以下6组作用:①IL-1β+IFN-γ组;②IL-1β+IFN-γ+DXM组;③IL-1β+IFN-γ+T4组;④T4组;⑤DXM组;⑥对照组(DMEM)。ELISA法检测培养上清IL-4、IFN-γ水平,硝酸还原酶法检测上清一氧化氮水平,化学发光法检测上清胰岛素水平。结果①IL-1β+IFN-γ刺激的NIT-1细胞分泌IL-4水平较其他各组减少(P<0.01),IFN-γ分泌比其他各组增多(P<0.01),IL-4/IFN-γ比其他各组降低(P<0.01)。②T4+IL-1β+IFN-γ刺激的NIT-1细胞与IL-1β+IFN-γ刺激组相比,分泌IL-4水平增高(P<0.01),IFN-γ减少(P<0.01),IL-4/IFN-γ增高(P<0.01)。③IL-1β+IFN-γ刺激的NIT-1细胞分泌NO较其他各组增高(P<0.01),胰岛素分泌减少(vsIL-1β+IFN-γ+T4组、IL-1β+IFN-γ+DXM组,P<0.05;vsT4组、DXM组、对照组,P<0.01)。④T4+IL-1β+IFN-γ刺激的NIT-1细胞与IL-1β+IFN-γ组相比,分泌NO水平减少(P<0.01),Ins增加(P<0.05);与IL-1β+IFN-γ+DXM组相比,NO分泌减少(P<0.05)。结论 T4可减轻IL-1β+IFN-γ对NIT细胞的损伤作用,保护NIT细胞的胰岛素分泌功能。
Objective To investigate the protective effect of Tripterygium Hypoglaucum (Thunbergii) T4 on pancreatic islet β cells and to provide experimental evidence for the immunoprotection of islet β cells. Methods Cultured NIT cells of islet β cell line and normal control peripheral blood mononuclear cells, respectively, with the following six groups: ① IL-1β + IFN-γ group; ②IL-1β + IFN-γ + DXM group; ③IL- γ + T4 group; ④ T4 group; ⑤ DXM group; ⑥ control group (DMEM). The levels of IL-4 and IFN-γ in the culture supernatant were detected by ELISA, the level of nitric oxide in the supernatant was detected by nitrate reductase method, and the level of insulin in the supernatant was detected by chemiluminescence. Results ① The level of IL-4 secreted by IL-1β + IFN-γ in NIT-1 cells was significantly lower than that in other groups (P <0.01) -γ was lower than other groups (P <0.01). (2) Compared with IL-1β + IFN-γ stimulation group, NIT-1 cells stimulated by T4 + IL-1β + IFN-γ increased IL-4 secretion and IFN- IL-4 / IFN-γ increased (P <0.01). (3) IL-1β + IFN-γ + DXM secreted by NIT-1 cells stimulated by IL-1β + IFN-γ increased compared with other groups (P <0.01) Group, P <0.05; vsT4 group, DXM group, control group, P <0.01). Compared with IL-1β + IFN-γ group, NIT-1 cells stimulated by T4 + IL-1β + IFN-γ reduced the levels of NO secreted by insulin and increased the levels of Ins (P <0.01) Compared with IFN-γ + DXM group, NO secretion decreased (P <0.05). Conclusions T4 can reduce the injury of NIT cells induced by IL-1β + IFN-γ and protect the insulin secretion of NIT cells.