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WRKY蛋白参与植物对生物或非生物胁迫反应和一些发育、代谢过程,在植物中组成一个转录因子大家族.从水稻cDNA文库中分离到一个新的WRKY基因——OsWRKY52cDNA,包括一个1719bp的开放读码框,推测编码一个由572个氨基酸组成的蛋白质,与燕麦(Avenasativa)AsWRKY1具有54%的氨基酸一致性.该基因被非亲和性稻瘟菌快速诱导.凝胶阻滞实验结果表明,原核表达的OsWRKY52能与水稻PR1a启动子上的W盒元件特异结合.采用酵母单杂交体系的方法证明了OsWRKY52具有转录激活活性,其丝氨酸岛、苏氨酸岛和C端的富酸性氨基酸区是负责转录激活的区域.这些结果提示OsWRKY52作为一个转录激活子,可能参与植物对稻瘟菌的应答反应.
The WRKY protein is involved in the biological and abiotic stress response of plants and some developmental and metabolic processes, forming a large family of transcription factors in plants. A new WRKY gene, OsWRKY52cDNA, was isolated from rice cDNA library and consisted of a 1719bp open reading Encoded a protein consisting of 572 amino acids and had 54% amino acid identity with AsWRKY1 of Avenasativa.The gene was rapidly induced by non-affinity Magnaporthe grisea.The results of gel retardation assay showed that prokaryotic The expressed OsWRKY52 specifically binds to the W box element on the rice PR1a promoter.The single-hybrid yeast system demonstrated that OsWRKY52 has a transcriptional activation activity and that its serine, threonine and C-terminal acidic amino acid regions are responsible for transcription Activated region.These results suggest that OsWRKY52, as a transcriptional activator, may be involved in plant response to Magnaporthe grisea.