论文部分内容阅读
为了提高木霉菌株几丁质酶的产量,采用单因素和正交试验设计,优化哈茨木霉Th-30生产几丁质酶的发酵条件,并就木霉几丁质酶粗提液对番茄灰霉病菌的拮抗作用进行了探讨。结果表明,哈茨木霉Th-30以7.0g/L几丁质、2.0g/L蛋白胨、50mL/L营养盐溶液、1.0mL/L微量元素为培养基,在起始pH5.5、温度28℃、装瓶量50mL/250mL、接种量8%的条件下,诱导96h获得的几丁质酶活性最高。哈茨木霉Th-30几丁质酶粗提液对番茄灰霉病菌有明显的拮抗作用,在稀释2倍的情况下,对番茄灰霉病菌生长的抑制率为67.61%～71.31%,对灰霉病菌孢子产生及萌发的抑制率分别为80.95%和81.35%,对病菌孢子致病性的抑制率为65.22%～70.51%。
In order to improve the yield of chitinase in Trichoderma strains, the fermentation conditions of Chitinase production by Th. Trichoderma Th-30 were optimized by single factor and orthogonal design. The effects of Trichoderma chitinase crude extract on the growth of tomato Antagonism of Botrytis cinerea was discussed. The results showed that Trichoderma harzianum Th-30 with 7.0g / L chitin, 2.0g / L peptone, 50mL / L nutrient solution, 1.0mL / L trace elements as the medium, the initial pH5.5, temperature 28 ℃, bottling volume 50mL / 250mL, inoculation 8% of the conditions, induced 96h chitinase activity obtained the highest. Trichoderma harzianum Th-30 chitinase crude extract of Botrytis cinerea obvious antagonism, in the case of diluted 2 times, the inhibition of Botrytis cinerea growth 67.61% ~ 71.31%, gray The inhibitory rates of sporulation and germination of molds were 80.95% and 81.35%, respectively, and the pathogenicity of molds was 65.22% -70.51%.