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目的:研究长苞凹舌兰提取物(CE)能否改善胆碱能损伤大鼠学习记忆的能力,及对胆碱乙酰化酶(ChAT)活性和表达水平的影响。方法:采用鹅蒿蕈氨酸基底前脑注射法建立拟痴呆大鼠模型。实验动物分为假手术组,模型组,CE 1.25和5 mg.kg-1组,连续ig 28 d。避暗实验检测大鼠学习记忆能力;[3H]标记实验测定脑组织ChAT的活性;免疫组化实验测定脑内ChAT的表达水平。结果:与模型组比,CE 5 mg.kg-1组大鼠在避暗实验中的潜伏期明显延长;大脑皮层的ChAT活性显著升高;额叶皮层和基底前脑ChAT表达水平升高。结论:CE可改善胆碱能损伤大鼠的学习记忆能力,其机制可能与提高ChAT活性和表达水平有关。
OBJECTIVE: To investigate whether the extract of E. militaris (CE) can improve the ability of learning and memory in rats with cholinergic injury and the effect on the activity and expression of choline acetylase (ChAT). METHODS: A rat model of dementia was established by using a basal forebrain injection of Artemisia gondii. Experimental animals were divided into sham operation group, model group, CE 1.25 and 5 mg.kg-1 groups, continuous ig 28 d. Dark learning was used to test the learning and memory ability of rats; [3H] labeling experiment was used to determine the activity of ChAT in brain tissue; immunohistochemistry was used to determine the expression level of ChAT in brain. RESULTS: Compared with the model group, the incubation period of the CE 5 mg.kg-1 group was significantly prolonged in the dark test; the ChAT activity in the cerebral cortex was significantly increased, and the expression of ChAT in the frontal cortex and basal forebrain was increased. Conclusion: CE can improve the learning and memory ability of rats with cholinergic injury. The mechanism may be related to the increase of ChAT activity and expression level.