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目的：探讨吗啡对小鼠血清一氧化氮(NO)的影响。方法：①吗啡急性用药实验：将小鼠随机分为5组(每组10只)，其中4组按100 me／kg腹腔注射盐酸吗啡，将小鼠在注射吗啡后0．5 h，1 h，2 h，4 h分批处死并收集血清，另1组注射生理盐水为对照组，在注射后1 h处死小鼠并收集血清。②吗啡成瘾性实验：另将小鼠随机分为5组(每组10只)，其中4组每d按100 me／kg注射盐酸吗啡，共注射10 d，在末次注射后6 h分别将吗啡成瘾组、纳络酮诱发戒断组小鼠处死并收集血清，其余2组小鼠在自然戒断1 d及6 d后处死并收集血清；另1组注射生理盐水为对照组，每d按0．5 ml／只注射生理盐水，共注射10 d，在末次注射后6 h后处死小鼠并收集血清。③采用硝酸还原酶法检测血清NO浓度。结果：①吗啡急性用药实验：在注射吗啡后0．5 h，1 h，2 h血清NO浓度分别为(121．09±16．66)μnol／L，(132．00±27．51)μnol／L，(127．35±13．μmol)umol／L，均显著高于对照组(86．62±7．87)μmol／L，(P0．05)。②吗啡成瘾性实验：吗啡成瘾组、纳络酮诱发戒断组及自然戒断1 d组血清NO浓度分别为(114．60±15．15)μmol／L，(122．12±22．73)μmol／L和(132．81±39．98)μnol／L，对照组为(92．14±14．98)μnol／L，3组结果均高于对照组(P<0．05，P<0．01，P0．05)。结论：注射吗啡及吗啡成瘾和戒断可升高小鼠血清NO浓度。“,”Aim: To determine the effects of morphine hydrochloride on mice serum niwic oxide(NO) level. Methods: (①)Tests of acute injection of morphine:Mice were divided into five groups ( 10 of each group).Four groups were injected with mor-phine hydrochloride at dose of 100 mg/kg and serum was collected at 0.5 h, 1 h,2 h and 4 h for NO examination. The other groupwas injected with normal saline as control and serum was collected at 1h for NO examination. (②)Tests of morphine addiction:Othermice were divided into five groups( 10 for each group) . Four were injected with morphine hydrochloride at the dose of 100 mg/kgfor 10 days se that the mice were addicted to morphine . The other group was injected with normal saline at the dose of 0.5 ml permouse for 10 days as conntrol. Serum of morphine addicted group, morphine abstinence group were collected after 6 h of the last in-jection. Serum of the spontaneousness abstinence ld and 6 d groups were also collected. Serum of the control group was collectedafter 6 h of the last injection. (③)NO were examined by using nitric acid reductase method. Results: (①)Tests of acute injection ofmorphine:The values of serum NO were (121.09± 16.66)mmol/L, (132.00 ± 27.51)mmol/L and (127.35± 13.29)μmol/L at0.5 h,1 h and 2 h respectively after morphine injection, and of control group was (86.62 ± 7.87)μmol/L. There were statisticallydifference between each experimental and control group( P < 0.01 ). (2)Tests of morphine addiction: The values of serum NO ofmorphine addicted group , morphine abstinence group and spontaneousness abstinence 1 d group were (114. 60± 15. 15)mmol/L, (122. 12 ± 22. 73) mmol/L and (132. 81 ± 39. 88) μ. mol/L, which were higher than that of conurol group (92. 97 ±14. 98) μmol/L respectively (P<0. 05, P<0. 01, P<0. 01), and that of the spontaneousness abstinence 6 d grouphad no difference compared with that of control group. Conclusion: The levels of serum NO raised after morphine injection,morphine addiction and morphine abstinence.