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目的:观察腹腔感染大鼠肠屏障损伤后肠道通透性的改变与I-FABP表达的关系。方法:清洁级健康的成年雄性Wistar大鼠40只,随机分为空白对照组(仅行简单的剖腹手术)和腹腔感染(采用CLP盲肠结扎穿孔法制作腹腔感染模型)术后12 h、24 h、36h、48 h组,每组8只,相应时间点处死大鼠后,测定血浆D-乳酸和I-FABP含量。结果:腹腔感染后12 h,大鼠血浆D-乳酸含量开始增加(P<0.05),血浆I-FABP含量也开始增加(P<0.01);血浆D-乳酸含量在24 h达到最高值(P<0.01),血浆I-FABP含量在36h达到最高值(P<0.01)。大鼠血浆D-乳酸水平与I-FABP具有显著相关性(r=0.626,P<0.01)。结论:腹腔感染状态下,肠屏障严重受损,肠粘膜通透性的改变与血浆I-FABP水平具有显著的相关性。
Objective: To observe the relationship between intestinal permeability and I-FABP expression after intestinal barrier injury in rats with intraperitoneal infection. Methods: Forty healthy adult male Wistar rats were randomly divided into blank control group (simple laparotomy) and abdominal infection (CLP model of abdominal cecal infection) at 12 h, 24 h , 36h, 48h group, with 8 rats in each group. After the rats were sacrificed at the corresponding time points, the contents of D-lactate and I-FABP in plasma were determined. Results: After 12 h of intraperitoneal infection, the plasma D-lactic acid content began to increase (P <0.05) and the content of plasma I-FABP began to increase (P <0.01). The plasma D-lactic acid content reached the peak at 24 h <0.01). The content of plasma I-FABP reached the highest value at 36h (P <0.01). There was a significant correlation between plasma D-lactate level and I-FABP (r = 0.626, P <0.01). CONCLUSIONS: The intestinal barrier is severely impaired in peritoneal infection, and the change of intestinal mucosal permeability has a significant correlation with plasma I-FABP level.