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从哈密瓜上分离到两种引起坏死症状的病毒,称为哈密瓜坏死病毒(HmNV)和哈密瓜叶脉坏死病毒(H-mVNV).两者都能系统感染哈密瓜,响影植株滞长. HmNV可侵染葫芦科、豆科和茄科的14个品种,但是不侵染普通烟、苋色藜和千日红.T·D·P75—85℃,D·E·P·10~(-5)—10~(-6),L·I·V·7天.提纯的HmNV含有两种球形粒子,直径约为24nm和16nm.沉降常数为114S和73S.A260/A280比值为1.82,A260/A235比值为1.57.在琼脂糖双扩散试验中与烟草坏死病毒的抗血清发生沉淀反应,与甜瓜坏死斑病毒(MNSV)的抗血清无沉淀.根据这些性质,HmNV应是烟草坏死病毒的一个株系或是烟草坏死病毒组的一个成员。HmVNV的寄主范围局限在葫芦科的黄瓜属,不侵染南瓜属的西葫芦,在供试的几个甜瓜品种上引起明显的叶脉坏死.此外,还系统侵染蚕豆,局部侵染苋色藜和千日红.T·D·P·55—60℃,D·E·P·10~(-3)—10~(-4),L·I·V·3—6天.病毒质粒约为13nm×660nm微弯曲的线条.桃蚜能从感病的甜瓜或蚕豆上获得病毒并传染给甜瓜.经琼脂凝胶双扩敏试经,HmVNV与西瓜花叶病毒1号和2号(WMV—1和WMV—2)的抗血清不产生沉淀线.
Two viruses that cause necrosis were isolated from the cantaloupe, called the Hami melon necrosis virus (HmNV) and the Hami melon vein virus (H-mVNV), both of which systematically infected cantaloupe and diastolic plants. Cucurbitaceae, Leguminosae and Solanaceae, but did not invade common smoke, amaranth, and amaranth. T · D · P75-85 ℃ · D · E · P · 10 -5 - 10 -6 and L · V · 7 days.The purified HmNV contained two types of spherical particles with diameters of about 24 nm and 16 nm.The settling constants were 114S and 73S.The A260 / A280 ratio was 1.82, the A260 / A235 ratio Was 1.57. Precipitation occurred in antiserum against tobacco necrosis virus in the agarose double-diffusion assay, with no precipitation from the antisera of melon necrotic spot virus (MNSV). According to these properties, HmNV should be a strain of tobacco necrosis virus or Is a member of the tobacco necrosis virus group. The host range of HmVNV was confined to cucurbitaceous Cucurbitaceae and not to Zucchini of Cucurbitaceae, causing obvious necrosis of veins in several melon cultivars tested.In addition, Vicia faba was also systematically infected, Thousand red .T P P 55-60 ℃, D · E · P · 10 -3 -10 -4, L · V · 3-6 days. The viral plasmid is about 13nm × 660nm micro-curved lines. Myzus persicae can get the virus from the infected melon or broad bean virus and transmitted to the melon by agar gel double-sensitivity test, HmVNV and watermelon mosaic virus 1 and 2 (WMV- 1 and WMV-2) did not produce a precipitate line.