论文部分内容阅读
最近,对于乙型肝炎表面抗原(HBsAg)和乙型肝炎e抗体(抗-HBe)阳性母亲所产婴儿是否需要进行被动或主动免疫存在着争论。由于有一定数量的抗-HBe阳性母亲可通过垂直传播将乙型肝炎病毒(HBV)传染给婴儿,因此作者对44例HBsAg阳性孕妇的血清进行了HBV-DNA检测,后者可作为HBV传播的一个指标。以克隆的HBV-DNA pCp10为探针进行斑点杂交试验。结果7例HBeAg阳性血清标本中有6例(86%)HBV-DNA呈强阳性,其中3例血清DNA多聚酶和HBV-DNA同时阳性;在33例抗-HBe阳性血清标本中也有8例(24%)可检出HBV-DNA;在4例HBeAg和抗-HBe(RIA法)均阴性的血清中有2例检出了HBV-DNA。未观察到HBeAg阴性血清中可检出DNA多聚酶活性。 HBsAg滴度(1∶10~3～1∶10~5)和转氨酶水平均
Recently, there has been debate about whether passive or active immunization of infants born to hepatitis B surface antigen (HBsAg) and hepatitis B e antibody (anti-HBe) positive mothers is required. Since a number of anti-HBe positive mothers were able to transmit hepatitis B virus (HBV) to infants by vertical transmission, the authors tested HBV-DNA in sera of 44 HBsAg-positive pregnant women who were transmitted as HBV An indicator. Dot blot hybridization was performed using the cloned HBV-DNA pCp10 as a probe. Results Of the 7 HBeAg positive serum samples, 6 (86%) were strongly positive for HBV-DNA, and 3 of them were positive for HBV DNA and DNA polymerase. Of the 33 anti-HBe positive serum samples, 8 (24 %) Detected HBV-DNA; HBV-DNA was detected in 2 out of 4 serums negative for both HBeAg and anti-HBe (RIA). No DNA polymerase activity was detectable in HBeAg-negative sera. HBsAg titer (1:10 ~ 3 ~ 1:10 ~ 5) and aminotransferase levels were