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目的探讨在人类多形性胶质母细胞瘤(GBM)中,变异型IκBα(IκBαM)基因对肿瘤发生发展的抑制作用及相应的作用机制。方法将经过IκBαM基因转染的人类GBM细胞植入裸鼠皮下制作异位肿瘤生长动物模型,检测肿瘤组织的发生及发展,进一步通过免疫组织化学染色方法分析肿瘤中的微血管密度。结果体内多形性胶质母细胞瘤的生长受到IκBαM基因的抑制,表现为不发生肿瘤或迟发,并且肿瘤生长缓慢,而且肿瘤组织中的微血管密度也显著降低。结论IκBαM基因对人类GBM具有生长抑制作用,并且血管形成减少是其作用机制中的重要环节。
Objective To investigate the inhibitory effect of mutated IκBα (IκBαM) gene on tumor development and its mechanism in human glioblastoma multiforme (GBM). Methods Human GBM cells transfected with IκBαM gene were subcutaneously implanted into nude mice to establish the model of ectopic tumor growth. The occurrence and development of tumor tissue were detected, and the microvessel density in tumor was further analyzed by immunohistochemical staining. Results The growth of glioblastoma multiforme in vivo was inhibited by the IκBαM gene, showing no tumor or late onset, slow tumor growth, and a significant decrease in microvessel density in tumor tissue. Conclusion The IκBαM gene inhibits the growth of human GBM, and the decrease of angiogenesis is an important part of its mechanism.