将供试的国内外大麦品种384个,种植于浙江嘉善县、江苏盐城市和上海市大麦黄花叶病抗性鉴定圃,以感病大麦品种早熟3号为阳性对照,无病田健株为阴性对照。在3月份大麦黄花叶病发病季节,每一品种选可疑株或随机采样品10株,每株采已充分展开的心叶1片,用F(ab’)_2-ELISA方法进行检测,根据检测结果与症状表现进行比较,探讨应用血清学方法大量检测大麦品种对黄花叶病抗性评价的可行性。 384 domestic and foreign barley cultivars were planted in Jiashan County of Zhejiang Province, Yancheng City of Jiangsu Province and Shanghai Yellow Mosaic Disease Resistance Identification Nursery. The susceptible barley cultivars precocious puberty 3 were positive control, Negative control. In the season of barley yellow mosaic disease in March, 10 strains of suspicious strains or random samples were selected for each variety, and 1 piece of heart leaf that had been fully developed was taken for each strain. F (ab ’) _2-ELISA method was used to detect the disease. Results and symptoms were compared to explore the application of serological methods to detect large quantities of barley varieties susceptible to yellow mosaic disease feasibility.