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用甲基胆蒽诱发的同系小鼠肉瘤1315或1321接种的带瘤小鼠和肿瘤免疫小鼠(即接种肉瘤后14天,手术切除肿瘤的小鼠)的脾脏和淋巴结的淋巴样细胞,在体外分别与用丝裂霉素 C 处理过的相应肉瘤细胞一起培养3天或6天,进行体外致敏。用微量细胞毒性试验的方法,比较这两种小鼠的淋巴样细胞经体外致敏后对靶细胞的细胞毒性。体外致敏3天和6天的免疫小鼠淋巴样细胞对靶细胞都有细胞毒活力,并且致敏6天者比3天者强。带瘤小鼠淋
Mice infected with syngeneic mouse sarcoma 1315 or 1321 induced with methylcholanthrene, and lymphoid cells of the spleen and lymph nodes of immunized mice and mice immunized with the tumor (ie, 14 days after inoculation of the sarcoma) In vitro sensitization was performed by in vitro culture with corresponding sarcoma cells treated with mitomycin C for 3 days or 6 days. The cytotoxicity of the lymphocyte-like cells of the two mice on the target cells after in vitro sensitization was compared by a method of micro cytotoxicity. In vitro sensitized mouse lymphoid cells 3 days and 6 days had cytotoxic activity on the target cells, and sensitized for 6 days was stronger than 3 days. Mice with tumor