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目的制备D4S1627、D14S1426、D1S1649 STR基因座的等位基因分型标准物,构建这3个STR基因座的复合扩增同步检测方法并进行遗传多态性分析。方法本实验采用复合扩增同步检测方法,聚丙烯酰胺凝胶电泳分离,银染显色,检测成都地区120名无关汉族个体的D4S1627、D14S1426、D1S1649 STR基因座基因型及等位基因频率分布。结果 3个STR基因座复合扩增同步检测电泳分型条带清晰,各等位基因片段互不重叠,每一个体样本与单个基因座检测结果一致。复合扩增同步检测方法结果显示,3个STR基因座在成都市汉族群体中的基因型分布符合Hardy-Weinberg平衡,基因座D4S1627检出6个等位基因(10～15);D14S1426检出9个等位基因(5、6、8～14),未检出等位基因7;D1S1649检出6个等位基因(7～12)。3个STR基因座的累积个人识别率为0.9989,累积非父排除率为0.9603。结论成功建立D4S1627、D14S1426和D1S1649 STR基因座复合扩增同步检测方法,这3个STR基因座在群体遗传研究以及法医学鉴定中具有实际应用价值。
Objective To prepare the allelic typing standards of D4S1627, D14S1426 and D1S1649 STR loci and to establish a multiplex amplification simultaneous detection method for these three STR loci and analyze the genetic polymorphism. Methods In this experiment, the genotypes and allele frequencies of D4S1627, D14S1426 and D1S1649 STR in 120 unrelated Han individuals in Chengdu area were detected by multiplex amplification and synchronous detection, polyacrylamide gel electrophoresis and silver staining. Results Three STR loci multiplex amplification synchronous detection electrophoresis typing bands clear, the allele fragments do not overlap, each individual sample with a single locus test results. The result of multiplex amplification and simultaneous detection showed that the genotype distribution of three STR loci in Han population of Chengdu accorded with Hardy-Weinberg equilibrium, six alleles (10-15) were detected in locus D4S1627, and 9 Alleles (5, 6, 8 to 14), no allele 7 was detected; D1S1649 detected 6 alleles (7 to 12). The cumulative personal recognition rate of three STR loci was 0.9989, and the cumulative non-parent exclusion rate was 0.9603. Conclusion The simultaneous detection of multiplex amplification of D4S1627, D14S1426 and D1S1649 STR loci has been successfully established. These three STR loci have practical value in population genetic research and forensic identification.