论文部分内容阅读
对采自辽东湾和韩国江华岛的2个海蜇群体56个个体的线粒体COI基因序列进行扩增,并结合Gen Bank上其他15个海蜇样品的同源序列对其进行序列变异分析,研究海蜇群体的遗传特征和群体遗传结构状况.在所分析的71个个体中共检测到28个多态位点,定义了32种单倍型.海蜇群体呈现出高的单倍型多样性(0.91±0.06~0.94±0.01)和中等或较低的核苷酸多样性[(0.60±0.34)%~(0.68±0.40)%].与其他几种大型水母相比,海蜇群体的遗传多样性水平较高.系统分析结果显示,海蜇群体间具有2个明显的单倍型谱系分支.比较海蜇不同群体间的遗传分化指数(F_(ST))和分子变异分析(AMOVA)发现,在本研究取样范围内不同海蜇群体间存在明显的遗传结构.不同海蜇群体存在复杂的遗传模式,海蜇独特的生活史特征、其分布海域的海流状况及人为因素如增殖放流等可能是造成海蜇这种复杂遗传模式的原因.
The mitochondrial COI gene sequences of 56 individuals from two jellyfish populations collected from Liaodong Bay and South Korea’s Ganghwa Island were amplified and sequenced by using the homology sequences of 15 other jellyfish samples from Gen Bank. Genetic characteristics and genetic structure of the population.A total of 28 polymorphic loci were detected in 71 individuals analyzed and 32 haplotypes were defined.The populations showed high haplotype diversity (0.91 ± 0.06 ~ 0.94 ± 0.01) and moderate or low nucleotide diversity [(0.60 ± 0.34)% ~ (0.68 ± 0.40)%]. Compared with several other jellyfish species, the genetic diversity of jellyfish population was higher. The results of phylogenetic tree analysis showed that there were 2 distinct haplogroup branches in the population of jellyfish.Comparing the genetic differentiation index (F_ (ST)) and molecular variance analysis (AMOVA) among different populations of jellyfish, There are obvious genetic structures among the populations of jellyfish.The complex genetic patterns of different jellyfish populations, the unique life history characteristics of jellyfish, the current situation in the sea area and the human factors such as multiplication and release may be the reason for the complex genetic model of jellyfish.