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目的调查山东省烟台地区发热伴血小板减少综合征疑似病例新型布尼亚病毒(SFTSV)感染情况。方法采用双重探针引物实时荧光定量-聚合酶链反应方法对发热伴血小板减少综合征疑似病例血清进行新型布尼亚病毒特异性核酸检测。结果 135份疑似病例血清样本中新型布尼亚病毒特异性核酸阳性81份,阳性率60.00%。发病第5～8天核酸检出阳性最高;发病高峰主要集中在5-8月;人群年龄分布在37～87岁之间,以60～70岁年龄组发病数最多,占总病例数的32.10%(26/81);92.59%(75/81)的患者为农民;男女性别比为1.79∶1。结论烟台地区发热伴血小板减少综合征疑似病例存在新型布尼亚病毒感染。
Objective To investigate the infection of novel Bunyavirus (SFTSV) in suspected cases of fever with thrombocytopenia in Yantai, Shandong Province. Methods The new Bunyavirus - specific nucleic acid was detected in serum of patients with fever and thrombocytopenia syndrome by double probe primer real - time fluorescence quantitative polymerase chain reaction. Results A total of 81 new Bunyavirus-specific nucleic acids were detected in 135 serum samples from suspected cases, the positive rate was 60.00%. The onset of the first 5 to 8 days the highest positive detection of nucleic acids; peak incidence mainly concentrated in the May-August; population age distribution between 37 to 87 years old, 60 to 70 age group the highest number of cases, the total number of cases 32.10 % (26/81); 92.59% (75/81) were farmers; sex ratio was 1.79:1. Conclusion There are new Bunyanoviruses in the suspected cases of fever with thrombocytopenia in Yantai.