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Obejctive To isolate genes related to blood glucose control using Sprague Dawley (SD) rat skeletal muscle Methods Differential gene expression between glucose stimulated and non glucose stimulated SD rat skeletal muscle was obtained by the differential display (DD) method, Slot blotting hybridization and Northern blot hybridization Results Several new genes that are differentially expressed in glucose stimulated and non glucose stimulated SD rat skeletal muscle were isolated 74 were verified by slot analysis from 181 gene tags isolated Of them, 33 were cloned and sequenced, and homologous analysis and application for GenBank Access Number were carried out 21 expressed sequence tag (EST) representing novel genes was confirmed by Northern blot analysis A total of 9 novel genes showed significant differential expression patterns Conclusions Using improvements and modifications of the differential display technique, a labor and cost saving route was used to identify new genes related to blood glucose control We investigated differentially expressed genes at the whole body level instead of the culture cell level, to ensure experimental results closer to the normal physiological state This technique may be valid in wide spread application to other related research
Obejctive To isolate genes related to blood glucose control using Sprague Dawley (SD) rat skeletal muscle Methods Differential gene expression between glucose stimulated and non glucose stimulated SD rat skeletal muscle was obtained by differential display (DD) method, Slot blotting hybridization and Northern blot hybridization Results Several new genes that are differentially expressed in glucose stimulated and non glucose stimulated SD rat skeletal muscle were isolated 74 were verified by slot analysis from 181 gene tags isolated them, 33 were cloned and sequenced, and homologous analysis and application for GenBank Access Number were carried out 21 expressed sequence tag (EST) presenting novel genes was confirmed by Northern blot analysis A total of 9 novel genes showed significant differential expression patterns Conclusions Using improvements and modifications of the differential display technique, a labor and cost saving route was used to identify n ew genes related to blood glucose control We were differentially expressed genes at the whole body level instead of the culture cell level, to ensure conclusions results closer to the normal physiological state This technique may be valid in wide spread application to other related research