论文部分内容阅读
我们应用维甲酸对人黑色素瘤细胞系的分化进行了实验研究。实验结果显示:维甲酸对黑色素瘤细也有一定的生长抑制作用,使瘤细胞倍增时间延长,裸鼠皮下移植瘤生长速度减缓,细胞形态亦随之发生变化,由上皮样细胞向梭形或树突状细胞转化。电镜下,诱导后的细胞细胞器较丰富.黑色素小体增多。免疫组化HLA-DR染色在未诱导细胞阳性,诱导后转阴。酪氨酸酶活性在诱导后呈增高趋势,r-src癌基因表达减少。上述结果支持维甲酸通过改变细胞表面的抗原性、癌基因及细胞内酶活性等多种途径发挥诱导分化作用。
We applied retinoic acid to the experimental study of the differentiation of human melanoma cell lines. The experimental results showed that: Retinoic acid also has a certain growth inhibitory effect on melanoma tumors, so that the doubling time of tumor cells is prolonged. The growth rate of subcutaneous xenografts in nude mice is slowed down, and the cell morphology also changes, from epithelial-like cells to spindle or tree. Confocal cell transformation. Under the electron microscope, the organelles after induction were more abundant and the melanosomes increased. Immunohistochemical HLA-DR staining was positive in non-induced cells and negative after induction. Tyrosinase activity increased after induction, and r-src oncogene expression decreased. The above results support that retinoic acid exerts differentiation-inducing effects through various pathways such as changing the cell surface antigenicity, oncogenes, and intracellular enzymatic activity.