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目的建立一种固相萃取-气相色谱法测定食用菌中22种有机磷农药残留的方法。方法食用菌被乙腈匀浆、超声、振荡提取,无水硫酸钠脱水,浓缩后经固相萃取柱净化,氮气吹干,以丙酮定容,再通过DM-17毛细管柱和PFPD检测器定性、定量。结果22种有机磷农药的分离效果好,线性相关系数均>0.99,最低检出限为0.000 18 mg/kg~0.035 mg/kg,平均回收率为68%~116%,RSD为2.1%~5.8%。结论该方法具有快速、简便、准确度和精密度好的优点,适合食用菌中多种有机磷农药残留的检测。
Objective To establish a method for the determination of 22 organophosphorus pesticide residues in edible fungi by solid-phase extraction and gas chromatography. Methods The edible fungi were homogenized with acetonitrile, extracted by sonication, extracted by shaking, dehydrated by anhydrous sodium sulphate, concentrated and purified by solid-phase extraction (SPE) column. Nitrogen was evaporated to dryness and fixed with acetone. Qualitative analysis was carried out by DM-17 capillary column and PFPD detector. Quantitative. Results The separation efficiencies of 22 organophosphorus pesticides were good with the linear correlation coefficients> 0.99, the lowest detection limit was 0.000 18 mg / kg ~ 0.035 mg / kg, the average recovery was 68% ~ 116% and the RSD was 2.1% ~ 5.8 %. Conclusion The method has the advantages of fast, simple, accurate and good precision, suitable for the detection of a variety of organophosphorus pesticide residues in edible fungi.