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比较10种大孔吸附树脂对板栗总苞中多酚的吸附与解吸性能。在静态吸附研究基础上,筛选较好的树脂做动态试验,并比较所得多酚与纯化前多酚对IEC-6细胞在不同条件下增殖能力的影响。结果表明:AB-8大孔吸附树脂纯化板栗总苞多酚的效果最好。上柱液质量浓度1 mg/mL,流速2 mL/min,上柱液体积400 mL,洗脱液乙醇质量分数70%,洗脱流速1 mL/min,洗脱液体积140 mL。在此条件下,多酚的吸附率和解吸率分别为87.2%和97.7%,板栗总苞多酚纯度由47.86%提高到81.67%。板栗总苞多酚对IEC-6细胞增殖有促进作用,且多酚纯化后比纯化前效果明显。
The adsorption and desorption properties of polyphenols from chestnut chest were compared with ten macroporous adsorption resins. On the basis of the static adsorption study, the better resin was screened for the dynamic test, and the effect of the obtained polyphenols and the purified polyphenols on the proliferation ability of IEC-6 cells under different conditions was compared. The results showed that AB-8 macroporous resin had the best effect on the purification of total chest polyphenols. The column volume of 1 mg / mL, the flow rate of 2 mL / min, on the column volume 400 mL, the eluent ethanol mass fraction 70%, the elution flow rate of 1 mL / min, the eluent volume of 140 mL. Under these conditions, the adsorption and desorption rates of polyphenols were 87.2% and 97.7%, respectively. The purity of total polyphenols in chestnut increased from 47.86% to 81.67%. Chestnut polyphenols promote the proliferation of IEC-6 cells, and the purification of polyphenols is more effective than before purification.