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目的 :探讨甲基强的松龙对脊髓损伤后c fos基因表达的影响。方法 :采用Allen s法制成大鼠脊髓损伤模型 ,动物随机分三组 :假手术组、手术组、术后给药组 (术后 5min腹腔注射甲基强的松龙3 0mg/kg ,假手术组和手术组给等量生理盐水以对照 )。分 3个时间点 (术后 1小时、2小时、4小时 )进行c fos免疫细胞化学法染色。结果 :损伤后FOS样免疫反应神经元主要分布在后角Ⅰ、Ⅱ层 ,手术组FOS样神经元与假手术组比较明显增多 ,而用药组较之手术组明显减少 (P<0 .0 0 1 )。结论 :脊髓损伤后脊髓c fos基因表达显著增多 ,甲基强的松龙能抑制损伤后c fos基因表达。
Objective: To investigate the effect of methylprednisolone on c fos gene expression after spinal cord injury. Methods: Rats were randomly divided into three groups: sham operation group, operation group and postoperative administration group (intraperitoneal injection of methylprednisolone 30mg / kg 5 min after operation, sham operation Group and operation group to the same amount of saline to control). C fos immunocytochemistry staining was performed at 3 time points (1 hour, 2 hours and 4 hours after the operation). Results: FOS-like immunoreactive neurons were mainly located in the posterior horn Ⅰ and Ⅱ layers after injury. FOS-like neurons in the operation group were significantly increased compared with the sham-operated group, while those in the treated group were significantly decreased compared with the operation group (P <0.05) 1 ). CONCLUSION: The expression of c fos gene in spinal cord increased significantly after spinal cord injury, and methylprednisolone can inhibit the expression of c fos gene after injury.