硒是生物体内一种必需的微量元素，缺硒会抑制细胞分化和细胞周期的进程、诱导细胞凋亡以及增强某些病毒的毒力。此外，硒缺乏是克山病的重要病因学说，也是心血管病的危险因素。基于体外细胞实验的低硒研究应用广泛，然而，其低硒条件的构建尚无明确统一的方法。故文中对体外低硒条件构建方法及其应用的研究进展进行综述，以期为后续相关研究提供借鉴。“,”Selenium is an essential trace element in many living organisms. Selenium deficiency inhibits cell differentiation and cell cycle progression, induces apoptosis and increases the virulence of certain viruses. In addition, selenium deficiency is an important etiology of Keshan disease and a risk factor for cardiovascular disease. Low selenium studies based on n in vitro cell experiments are widely used. However, there is currently no clear and unified way to establish n in vitro low-selenium conditions. In this paper, we have reviewed the progress on establishment of low-selenium conditions n in vitro and its applications, so as to provide reference for subsequent study.n