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用EDTA在pH12或大于12时滴定鈣,借鈣黄綠素或鈣黄綠素W作指示剂,当到达終点时仍然残留着明显的黄綠色螢光,但若干滴定之前加几滴0.01％
Calcium was titrated with EDTA at pH 12 or greater, with calcein or calcein W as an indicator, and significant yellow-green fluorescence persisted when the end point was reached, but several drops of 0.01%