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目的:探讨功能化纳米纤维支架(NFS)承载骨髓源性心肌干细胞(MCSCs)移植对大鼠心肌梗死(MI)后心功能恢复的影响,寻找一种更适合承载MCSCs治疗MI的可注射性生物材料。方法:通过单细胞克隆培养技术,从骨髓间充质干细胞中筛选具有心肌特异分化潜能的MCSCs,并固相合成自聚多肽。将24只雌性大鼠MI模型随机分为3组,即对照组、MCSCs移植组和支架承载MCSCs移植组,每组8只。细胞移植后4周,用M型超声心动图检测心功能恢复的情况。将心脏组织切片并进行Masson染色,利用图像分析检测胶原纤维的比率。采用原位荧光杂交法标记心肌组织中的Y染色体细胞,并检测心肌肌钙蛋白T(cTnT)的表达。结果:与MCSCs移植组相比,支架承载细胞移植组大鼠的心功能指数明显提高,胶原纤维比率明显减小,Y染色体阳性细胞数明显增多(P<0.05)。结论:支架承载MCSCs移植治疗MI,有利于移植细胞在受损心肌内的存活和向功能性心肌细胞分化,并可促进心功能恢复,其疗效优于单纯干细胞移植。
OBJECTIVE: To investigate the effect of functionalized nanofiber scaffold (NFS) -loaded bone marrow-derived stem cells (MCSCs) on the recovery of cardiac function after myocardial infarction (MI) in rats and to find out an injectable organism that is more suitable for carrying MI in MCSCs material. Methods: MCSCs with myogenic differentiation potential were selected from bone marrow mesenchymal stem cells by single cell cloning and culture. Twenty-four female MI models were randomly divided into three groups: control group, MCSCs transplantation group and MCSCs transplantation group, 8 in each group. Four weeks after cell transplantation, M-mode echocardiography was used to detect cardiac function recovery. Heart tissue was sectioned and subjected to Masson staining to detect collagen fiber ratio using image analysis. Y chromosome cells in myocardial tissue were labeled by in situ fluorescent hybridization and cardiac troponin T (cTnT) expression was detected. Results: Compared with MCSCs transplantation group, the cardiac function index of scaffold-bearing cell transplantation group was significantly increased, the ratio of collagen fibers was significantly reduced, and the number of Y-chromosome positive cells was significantly increased (P <0.05). Conclusion: The transplantation of MCSCs with scaffolds for the treatment of MI is beneficial to the survival of transplanted cells in the damaged myocardium and to the differentiation of functional cardiomyocytes, and promotes the recovery of cardiac function. The curative effect is better than that of simple stem cell transplantation.