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目的探讨不明原因复发性流产(URSA)与子宫内膜/早孕蜕膜组织HOXA-10基因表达的关系。方法采用原位杂交技术,以HOXA-10反义核苷酸为探针检测51例URSA患者及38例正常生育(NF)组的分泌期子宫内膜/早孕蜕膜组织HOXA-10 mRNA的表达水平,并以灰度阳性单位(PU值)表示。结果NF组子宫内膜分泌早期HOXA-10mRNA表达的PU值腺体为(6.66±0.11),间质为(6.76±0.15);分泌中期分别为(10.95±0.90)及(11.46±1.08);分泌晚期分别为(11.05±1.12)及(11.54±1.10);蜕膜组织分别为(11.55±1.14)及(11.93±1.92);分泌中晚期及蜕膜组织的PU值显著高于分泌早期。URSA组不同时期子宫内膜及早孕蜕膜组织中HOXA-10 mRNA的表达水平基本一致,无明显的分泌中晚期及早孕期峰,并且分泌中晚期及早孕蜕膜组织的PU值明显低于NF同期组。两组各期腺体和间质的PU值无显著差异。结论子宫内膜HOXA-10基因在分泌中晚期及早孕蜕膜组织中的高表达可能和孕卵着床及妊娠维持密切相关,其表达缺陷可能是导致URSA发生的重要原因之一。
Objective To investigate the relationship between unexplained recurrent spontaneous abortion (URSA) and endometrial / early pregnancy decidual HOXA-10 gene expression. Methods The expression of HOXA-10 mRNA in secretory endometrium and early pregnancy decidua in 51 URSA patients and 38 normal fertility (NF) groups was detected by in situ hybridization with HOXA-10 antisense oligonucleotide Level, and the unit of gray scale (PU value) said. Results The mRNA expression of HOXA-10 in the early stage of endometrial secretion was (6.66 ± 0.11) in the NF group, and (6.76 ± 0.15) in the secretory endometrium. The secretion of HOXA-10 mRNA was (10.95 ± 0.90) and (11.46 ± 1.08) (11.05 ± 1.12) and (11.54 ± 1.10) in the late stage, and (11.55 ± 1.14) and (11.93 ± 1.92) in the decidua respectively. The PU value in the late and decidual tissues was significantly higher than that in the early stage of secretion. The expression level of HOXA-10 mRNA in URSA group at different stages of endometrium and early pregnancy decidua was basically the same, there was no obvious peak of secretion of middle and late pregnancy and early pregnancy, and the PU value of secreting advanced decidua was significantly lower than that of NF group. There was no significant difference in PU value between the two groups of glands and stroma. Conclusion The high expression of endometrial HOXA-10 gene in the late and early stage of decidual tissue secretion may be closely related to the implantation of the pregnant egg and the maintenance of pregnancy. The expression of HOXA-10 may be one of the important causes of URSA.