1985年10月,在湘西北对1例人工感染的无免疫力间日疟病例,从周围血液见虫的第8d、临床发作的第3d起,以驯化的同龄中华按蚊为媒介,每8h作1次离体吸血感染,连续144h,相当于3个红内裂体增殖周期。在血餐的第6～7d解剖蚊胃,记录卵囊的感染率和感染度。同时在整个实验过程中每2h测量1次体温,以便分析配子体活力周期与临床发作周期的关系。 In October 1985, one case of Artemisia malariae of immunity was infected in the northwestern part of Hunan Province. From the 8th day after the blood was seen in the peripheral blood and from the 3rd day of the clinical attack, an anopheles sinensis of the same age was used as a medium, every 8h For 1 off-body vampire infection, continuous 144h, equivalent to 3 red inner lobules proliferation cycle. Mosquitoes were dissected from 6th to 7th day of blood meal to record the infection rate and infection rate of oocysts. At the same time, body temperature was measured every 2h during the whole experiment to analyze the relationship between gametocyte vitality cycle and clinical attack cycle.