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目的:研究人参皂苷Rg3对人食管癌细胞生长的抑制作用及其机制。方法:MTT法检测人参皂苷Rg3对人食管癌细胞Eca9706增殖的抑制,用流式细胞仪分别检测细胞周期和凋亡情况。结果:在25~200μg/ml范围内,不同浓度人参皂苷Rg3能抑制Eca9706细胞增殖,且呈剂量依赖性。53μg/ml、86μg/ml、141μg/ml人参皂苷作用Eca9706细胞48h,细胞周期在S期的比例明显增多,细胞凋亡率明显增加。结论:人参皂苷Rg3可能通过干预细胞周期和凋亡对食管癌Eca9706细胞增殖起抑制作用。
Objective: To study the inhibitory effect of ginsenoside Rg3 on human esophageal cancer cell growth and its mechanism. Methods: The inhibitory effect of ginsenoside Rg3 on the proliferation of human esophageal carcinoma cell line Eca9706 was detected by MTT assay. Cell cycle and apoptosis were detected by flow cytometry. Results: In the range of 25 ~ 200μg / ml, different concentrations of ginsenoside Rg3 could inhibit the proliferation of Eca9706 cells in a dose - dependent manner. Eca9706 cells were treated with 53μg / ml, 86μg / ml and 141μg / ml ginsenosides for 48h, the cell cycle increased significantly in S phase and the apoptosis rate increased significantly. Conclusion: Ginsenoside Rg3 may inhibit the proliferation of esophageal carcinoma Eca9706 cells through the intervention of cell cycle and apoptosis.