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The separation stability under high-humidity is significant in practical applications for air filters. Herein, hydro-phobic polyvinyl chloride (PVC) nanofiber filters with bead-on-string structure are designed to steadily remove particle matter under high relative humidity of 90%–95%. The developed hydrophobic filters possess comparable separation performance with the hydrophilic one, but greatly enhanced stability. After the introduction of bead-on-string structure, the filtration performance can be furtherly improved due to the formed large cavities and hy-drophobicity. Such hydrophobic PVC filters can be promising candidates for air purification in practical applications especially in wet seasons.