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目的:研究白藜芦醇与眼镜蛇神经毒素在生理pH条件下的经光诱发产生的相互作用及其对镇痛的影响。方法:采用紫外可见光谱和荧光光谱法扫描不同白藜芦醇浓度下眼镜蛇神经毒素(NT)在pH7.4磷酸缓冲液中的光谱,测定365 nm光照射白藜芦醇后的NT的荧光光谱。采用醋酸扭体法和热板法考察在白藜芦醇和NT联合用药并经尾静脉光照后对小鼠的镇痛效果。结果:紫外可见光谱和荧光光谱峰形发生了变化,紫外可见光吸收增强,荧光猝灭,光照加剧了猝灭效应,说明NT与白藜芦醇产生了聚合作用。其结合常数Ka为5.12×105 L·mol-1,结合位点数n为1,结合距离R为3.40 nm,故易发生非辐射能量转移。白藜芦醇和NT联合给药并光照后,小鼠镇痛效果较单独给药或联合给药但非光照组增强。结论:白藜芦醇在光诱导下活化与NT聚合,产生了协同镇痛作用。
OBJECTIVE: To investigate the light-induced interaction between resveratrol and cobra neurotoxin at physiological pH and its effect on analgesia. Methods: Spectra of NT neurotoxin (NT) in phosphate buffered saline (pH 7.4) at different resveratrol concentrations were scanned by UV-visible spectroscopy and fluorescence spectroscopy. Fluorescence spectra of NT after 365 nm light exposure to resveratrol . The acetic acid writhing method and hot plate method were used to investigate the analgesic effects of resveratrol and NT combined with light on tail vein. Results: The UV-vis spectra and fluorescence spectra showed a change in their peak shapes. The absorption of UV-visible light was enhanced and the fluorescence quenched. The quenching effect was exacerbated by light. It indicated that NT and resveratrol produced polymerization. The binding constant Ka is 5.12 × 105 L · mol-1, the number of binding sites n is 1 and the binding distance R is 3.40 nm, so non-radiative energy transfer easily occurs. Resveratrol and NT combined administration and light, the mice analgesic effect compared with single administration or combination but non-light group increased. CONCLUSIONS: Resveratrol is activated by light-induced polymerization with NT, resulting in a synergistic analgesic effect.