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目的探讨miR-335在胶质瘤中的表达及其对胶质瘤增殖侵袭的影响。方法用实时荧光定量PCR方法检测39例胶质瘤组织及其对应癌旁组织miR-335的表达;利用Lipofect AMINETM2000将miR-335模拟物或抑制剂及其阴性对照转染至人U87胶质瘤细胞,分别应用CCK-8法和Transwell小室法检测人胶质瘤U87细胞的增殖及侵袭能力。结果胶质瘤组织中miR-335的相对表达量为0.93±0.09,癌旁组织的miR-335相对表达量为0.33±0.02,胶质瘤组织中miR-335的表达显著低于癌旁组织(P<0.01)。与对照组相比,miR-335模拟物能够显著抑制胶质瘤U87细胞系的增殖以及侵袭能力(P<0.01);miR-335抑制剂则能够显著上调胶质瘤U87细胞系的增殖以及侵袭能力(P<0.01)。结论 miR-335在胶质瘤组织中是低表达的,在胶质瘤U87细胞中过表达miR-335能够抑制细胞的增殖及侵袭能力。
Objective To investigate the expression of miR-335 in gliomas and its effect on the proliferation and invasion of glioma. Methods Real-time fluorescence quantitative PCR was used to detect the expression of miR-335 in 39 glioma tissues and their corresponding paracancerous tissues. The miR-335 mimics or inhibitors and their negative controls were transfected into human U87 glioma by Lipofect AMINETM2000 Cells were detected by CCK-8 method and Transwell chamber assay of human glioma U87 cell proliferation and invasion. Results The relative expression of miR-335 in glioma tissues was 0.93 ± 0.09, and the relative expression of miR-335 in adjacent tissues was 0.33 ± 0.02. The expression of miR-335 in glioma tissues was significantly lower than that in paracancerous tissues P <0.01). Compared with the control group, miR-335 mimics significantly inhibited the proliferation and invasion of glioma U87 cell line (P <0.01); miR-335 inhibitor significantly increased the proliferation and invasion of glioma U87 cell line Ability (P <0.01). Conclusion miR-335 is low expressed in glioma tissues. Overexpression of miR-335 in glioma U87 cells can inhibit the proliferation and invasion ability of glioma cells.