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目的:研究原发性肝细胞癌组织和癌旁组织中存活素(Survivin)、磷酸化Akt(p-Akt)和雌激素受体(ER)表达及意义。方法:应用蛋白质印迹法检测52例原发性肝细胞癌和8例正常癌旁组织中Survivin、P-Akt和ER的表达。结果:肝细胞癌组织中Survivin和p-Akt表达明显高于癌旁组织,Survivin和p-Akt的表达与肝癌患者术后复发呈阳性相关(P<0.05),且两者之间的表达也呈阳性相关(r=0.736,P<0.01),而ER在两种组织中表达相仿,与肝癌患者术后复发无相关性,P>0.05。多因素分析结果显示,肿瘤转移、Survivin和p-Akt阳性表达可作为肝癌复发的风险因子。结论:Survivin和p-Akt与原发性肝癌的发生发展关系极为密切,Survivin和p-Akt高表达者预后差,可作为肝癌预后的预测因子。
Objective: To investigate the expression and significance of survivin, p-Akt and estrogen receptor (ER) in primary hepatocellular carcinoma and adjacent normal tissues. Methods: The expressions of Survivin, P-Akt and ER in 52 cases of primary hepatocellular carcinoma and 8 cases of normal paracancerous tissues were detected by Western blotting. Results: The expression of Survivin and p-Akt in hepatocellular carcinoma was significantly higher than that in paracancer tissues. The expression of Survivin and p-Akt was positively correlated with the recurrence of hepatocellular carcinoma (P <0.05), and the expression of Survivin and p- (R = 0.736, P <0.01). However, the expression of ER was similar in both tissues and had no correlation with recurrence of HCC (P> 0.05). Multivariate analysis showed that the positive rates of tumor metastasis, Survivin and p-Akt could be used as risk factors for the recurrence of HCC. Conclusion: Survivin and p-Akt are closely related to the occurrence and development of primary hepatocellular carcinoma. Survivin and p-Akt overexpression have poor prognosis and may be used as predictors of the prognosis of hepatocellular carcinoma.