论文部分内容阅读
目的:探讨不同时间应用一氧化氮(nitric oxide,NO)前体左旋精氨酸(L-arginine,L-Arg)对大鼠学习记忆功能及大脑皮质和海马α7烟碱型乙酰胆碱受体(nicotinic acetylcholine receptor,α7 nAChR)表达的影响。方法:Wistar雄性大鼠随机分为6组,分别为连续3、7和14 d侧脑室注射L-Arg(0.5μmol/d,每日1次)的3个实验组,和在相同时间给予同剂量生理盐水的3个对照组。用Y型迷宫刺激器对大鼠进行学习和记忆能力的行为学检测;用NO试剂盒和免疫组化分别检测大鼠大脑前额叶皮质和海马NO含量以及α7 nAChR的表达。结果:大鼠的学习和记忆行为能力、大脑前额叶皮质和海马NO含量以及α7 nAChR阳性细胞数,在三个实验组分别与各自对照组比较均明显增加;在3个实验组之间比较,7 d组较3 d组,14 d组较7 d组比较均明显增加。3个时间点的对照组之间相比较上述指标均没有明显差异。结论:大鼠学习记忆行为改善以及大脑前额叶皮质和海马NO含量和α7 nAChR的表达随着应用L-Arg的延长而增加,NO对学习记忆的影响具有时间依赖性。
AIM: To investigate the effects of L-arginine (L-arginine), a nitric oxide (NO) precursor, on the learning and memory abilities of rats and on the nicotinic α7 nicotinic acetylcholine receptors acetylcholine receptor, α7 nAChR) expression. Methods: Wistar male rats were randomly divided into 6 groups: 3 experimental groups injected intracerebroventricularly with L-Arg (0.5μmol / d, once daily) for 3, 7 and 14 days respectively, and the same experimental group Three control groups of doses of normal saline. The behavior of learning and memory ability of rats was detected by Y-type maze stimulator. The content of NO and the expression of α7 nAChR in rat prefrontal cortex and hippocampus were detected by NO kit and immunohistochemistry respectively. RESULTS: The learning and memory abilities of rats, NO content in the prefrontal cortex and hippocampus, and the number of α7 nAChR positive cells in the three experimental groups were significantly increased compared with the respective control groups; in the three experimental groups, 7 d group than the 3 d group, 14 d group compared with the 7 d group were significantly increased. There was no significant difference between the above three indexes in the control group at 3 time points. Conclusion: The improvement of learning and memory behaviors and the expression of NO and α7 nAChR in prefrontal cortex and hippocampus of rats increased with the prolongation of L-Arg. The effect of NO on learning and memory was time-dependent.