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耳硬化症患者出现感觉神经性聋的发病机制有多种理论。其中有一说认为可能由于某种组织分解产物,如缩氨酸,排入外淋巴液产生对毛细胞的毒害。为验证此说,作者选择气-骨导差为20～30dB、骨导平均阈值为30dB的耳硬化症患者,在其镫骨手术时,切取硬化的镫骨底板、底板上层结构以及颞骨的骨皮质,立即冷藏于零下20°,用氯化胍(0.25M guanidine chloride)和乙二胺四醋酸(0.5M ethylene-dia minetetraacetic acid)溶液浸泡提取浸膏。所提取的非胶原缩氨酸/蛋白质用色层分析法分析。骨标本所含缩氨酸的成分用毛细管等速泳动对比分析。结果:耳硬化症的镫骨底板的等速泳动有13个带,镫骨的
There are many theories about the pathogenesis of sensory deafness in patients with otosclerosis. One said that may be due to some kind of tissue decomposition products, such as peptides, into the perilymph to produce hair cells poisoning. To verify this, we selected patients with otosclerosis who had a gas-bone conduction difference of 20-30 dB and a mean bone conduction threshold of 30 dB. During the tarsal surgery, the hardened tarsal floor, floor superstructure, and temporal bone Cortex, immediately refrigerated at minus 20 °, with guanidine chloride (0.25M guanidine chloride) and ethylenediaminetetraacetic acid (0.5M ethylene-dia minetetraacetic acid) solution soaking extract. Extracted non-collagenous peptides / proteins were analyzed by chromatography. The contents of peptide contained in the sample of bone were analyzed by isokinetic capillary electrophoresis. RESULTS: The otosclerosis of the stapes floor had 13 bands with tarsal motion