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肝细胞癌（ＨＣＣ）肿瘤抑制基因ｐ５３的改变有着显著的地理差异。应用地高辛标记探针原位杂交，ＰＣＲ、ＰＣＲ－ＳＳＣＰ分别检测重庆地区（ＨＢＶ高感染、黄曲霉素中等含量区）３８例ＨＣＣ中ＨＢＶＤＮＡ及ｐ５３基因突变，并应用Ｓｏｕｔｈｅｒｎ杂交对其中的１２例进行限制性片段多态性分析。结果显示３８例ＨＣＣ中３０例ＨＢＶＤＮＡ阳性（７９％）；９例ＨＣＣ有ｐ５３突变，其中８例ＨＢＶＤＮＡ阳性（８８．９％，Ｐ＞０．０５）。１２例ＨＣＣ中２例表现杂合性缺失（ＬＯＨ，１６．７％）．２例ＨＢＶＤＮＡ皆为阳性，占ＨＢＶＤＮＡ阳性例数的２０％（Ｐ＞０．０５）。结果表明，本地区ＨＣＣ与ＨＢＶ感染密切相关，ＨＢＶ可能与黄曲霉素协同作用，共同引起ｐ５３的改变，进而在ＨＣＣ发生发展过程中发挥重要作用。
The change of hepatocellular carcinoma (HCC) tumor suppressor gene p53 has significant geographical differences. Using in situ hybridization with digoxigenin labeled probes, PCR and PCR-SSCP were used to detect HBV DNA and p53 gene mutations in 38 HCC patients in Chongqing (highly infected HBV and moderate aflatoxin area), and Southern blotting was used to detect the mutations. Restriction fragment polymorphism analysis was performed in 12 patients. The results showed that 30 of 38 cases of HCC were positive for HBV DNA (79%); 9 cases of HCC had p53 mutations, of which 8 cases had positive HBVDNA (88.9%, P>0.05). Of the 12 cases of HCC, 2 showed loss of heterozygosity (LOH, 16.7%). Both cases were positive for HBV DNA, accounting for 20% of the HBV DNA positive cases (P>0.05). The results showed that HCC in this area is closely related to HBV infection, and HBV may act synergistically with aflatoxin, which causes changes in p53 and further plays an important role in the development of HCC.