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目的:观察miRNA-192在骨肉瘤中与各临床要素之间的关联,分析其与骨肉瘤患者生存情况之间的关系,为研究miRNA-192在骨肉瘤中的作用及可能的临床应用提供理论依据。方法:选取50例骨肉瘤新鲜样本组织及瘤旁组织,使用荧光RT-PCR的方法对样本中的miRNA-192的含量进行检测,结合详实的随访资料,分析miRNA-192与性别、年龄等临床要素之间的关系。通过生存分析的方法,分析miRNA-192与患者生存情况之间的关系。结果:miRNA-192与患者的年龄,肿瘤大小,分期,生存时间等有明显的统计学关联(P<0.05),包括年龄(P=0.028),临床分期(P=0.007),远端转移(P=0.013)和生存率(P=0.001)。结论:在临床样本中,miRNA-192与患者的多项临床要素有显著性关联,包括年龄,临床分期,远端转移和生存率,为接下来关于miRNA-192在骨肉瘤中的各项研究,特别是作为生物标记信号的临床应用奠定了良好的实验基础。
OBJECTIVE: To observe the relationship between miRNA-192 and various clinical factors in osteosarcoma, and to analyze the relationship between miRNA-192 and the survival of patients with osteosarcoma. To provide theoretical basis for studying the role of miRNA-192 in osteosarcoma and possible clinical application in accordance with. Methods: Fifty specimens of fresh osteosarcoma and para-tumor tissue were selected. The content of miRNA-192 in the sample was detected by fluorescence RT-PCR. Combined with detailed follow-up data, miRNA-192 was analyzed in relation to gender and age The relationship between the elements. Survival analysis was used to analyze the relationship between miRNA-192 and patient survival. Results: There was a significant correlation between miRNA-192 and age, tumor size, stage and survival time (P <0.05), including age (P = 0.028), clinical stage (P = 0.007), distant metastasis P = 0.013) and survival (P = 0.001). Conclusions: In clinical samples, there are significant correlations between miRNA-192 and multiple clinical features in patients, including age, clinical stage, distant metastasis and survival rate. In the next study on miRNA-192 in osteosarcoma , Especially as a biomarker signal clinical application has laid a good experimental basis.