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作者用经典方法制备出多层脱水-再水化囊泡(DRV)脂质体和多层囊泡(MLV)脂质体,研究了其组成成份、磷脂的胶-液相结晶变换温度(Tc)以及结合抗原的模式等多种参数,并根据用ELISA检测免疫后BALB/c小鼠血清中特异性IgG_1和IgG_(2b)的结果,探讨了脂质体特性对免疫纯破伤风类毒素的免疫应答的影响。用等克分子的胆固醇和多种卵磷脂衍生物作膜材,对DRV脂质体内载抗原(磷脂:类毒素重量比为24.7∶1)和MLV脂质体表面联结抗原(磷脂:类毒素为38.7∶1)的免疫佐剂作用的研究表明:以0.025μg和0.1μg两种
The authors prepared multilayer dehydrated-rehydrated vesicles (DRV) liposomes and multilamellar vesicles (MLV) liposomes using classical methods, and studied the composition, phospholipid gel-liquid crystal transformation temperature (Tc ) And antigen binding patterns and other parameters. Based on the results of ELISA for the detection of the specific IgG1 and IgG2 (2b) in BALB / c mice serum after immunization, the effects of liposome characteristics on the immunogenicity of tetanus toxoid Impact of immune response. Using equimolar cholesterol and a variety of lecithin derivatives as the membrane material, the anti-DRV liposomes (phospholipid: toxoid weight ratio of 24.7: 1) and MLV liposome surface antigen (phospholipids: toxoid 38.7: 1) showed that both 0.025μg and 0.1μg