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目的观察脑缺血再灌注时鼠脑纹状体区细胞外液（ＥＣＦ）多巴胺（ＤＡ）、去甲肾上腺素（ＮＥ）、５－羟色胺（５－ＨＴ）及其代谢产物高香草酸（ＨＶＡ）和５－羟吲哚乙酸（５－ＨＩＡＡ）的动态变化。方法采用脑内微透析技术，用高压液相色谱测定了前脑缺血３０ｍｉｎ、再灌注１２０ｍｉｎ时ＥＣＦＤＡ、ＮＥ和５－ＨＴ的变化。结果脑缺血１０ｍｉｎ时ＥＣＦＤＡ、ＮＥ迅速升高为缺血前的２８２和９１４倍，持续整个缺血期，再灌注后迅速下降达缺血前水平。脑缺血期ＨＶＡ、５－ＨＩＡＡ迅速下降，在缺血３０ｍｉｎ时达缺血前的４５％和５２％，再灌注后升高并在再灌注后６０ｍｉｎ，９０ｍｉｎ达缺血前的１５０％、１１３％。结论单胺类介质代谢紊乱参与了缺血性神经元损害
Objective To observe the changes of extracellular matrix (EC) dopamine (DA), norepinephrine (NE), serotonin (5-HT) and their metabolites homovanillic acid (HVA ) And 5-hydroxyindoleacetic acid (5-HIAA). Methods The intracerebral microdialysis technique was used to measure the changes of ECFDA, NE and 5-HT after 30min ischemia and 120min reperfusion in forebrain by high pressure liquid chromatography. Results ECFDA and NE rapidly increased to 282 and 914 times of pre-ischemic preconditioning at 10 min after cerebral ischemia, respectively. They continued throughout the ischemic period and rapidly dropped to their pre-ischemic levels after reperfusion. The number of HVA and 5-HIAA in cerebral ischemic phase decreased rapidly, reaching 45% and 52% before ischemia at 30min and increased after reperfusion, reaching 150% before ischemia at 60min and 90min after reperfusion %. Conclusion The monoaminergic metabolic disorder is involved in ischemic neuronal damage