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研究提示,链球菌糖蛋白(SAGP)诱发的瘤细胞生长抑制可能是通过其SH组与靶细胞上的未知受体相互作用实现的。这些作用所产生的信号经细胞内的转导通道引发细胞生长抑制。为弄清这一推测,首次就SAGP对靶细胞大分子合成的作用进行了研究。 由化脓性链球菌浸出物提纯巯基糖蛋白。选用鼠纤维肉瘤MethA细胞系(MethA)作为靶细胞。①SAGP对核酸前体与MethA细胞结合的作用:先以SAGP孵育MethA细胞,之后用~3H-胸腺嘧啶核苷或~3H-尿核苷脉冲。结果SAGP以浓度依赖方式抑制两种放
Studies have suggested that growth inhibition of tumor cells induced by streptococcal glycoprotein (SAGP) may be achieved through the interaction of its SH group with unknown receptors on target cells. The signals produced by these effects initiate cell growth inhibition via intracellular transduction channels. In order to clarify this hypothesis, the effect of SAGP on the macromolecular synthesis of target cells was studied for the first time. Purify the thioglycoprotein from Streptococcus pyogenes extract. The mouse fibrosarcoma MethA cell line (MethA) was used as a target cell. The effect of 1SAGP on the binding of nucleic acid precursors to MethA cells: MethA cells were first incubated with SAGP and then pulsed with ~3H-thymidine or ~3H-uridine. As a result, SAGP inhibits both types in a concentration-dependent manner.