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旨在探究酰基载体蛋白在植物脂肪酸合成途径中的作用机制,本研究采用传统PCR方法从花生中克隆得到了一个线粒体型基因,命名为AhmtACP3。并采用荧光定量PCR技术分析了基因的表达特性。该基因全长为859 bp,开放阅读框ORF为390 bp,开放阅读框对应的基因组序列为543 bp,由2个外显子和1个内含子组成,该基因编码129个氨基酸,理论分子量为14.5345 k D,理论等电点为5.22,可能定位于线粒体上。系统发育分析表明,花生线粒体型ACP蛋白分成2个分支:AhmtACP1和AhmtACP2有较近的亲缘关系,与AhmtACP3亲缘关系较远。荧光定量PCR结果显示,AhmtACP3基因在花中的表达量明显高于其他组织,其次是子叶和根。AhmtACP3基因在种子发育过程中的表达量呈现下降趋势。
In order to explore the mechanism of acyl carrier protein in plant fatty acid synthesis pathway, a mitochondrial gene named as AhmtACP3 was cloned from peanut using traditional PCR method. The gene expression characteristics were analyzed by fluorescence quantitative PCR. The full length of the gene was 859 bp, the open reading frame ORF was 390 bp, the open reading frame corresponding genomic sequence was 543 bp, which consisted of two exons and one intron, which encoded 129 amino acids. The theoretical molecular weight Is 14.5345 kD, the theoretical isoelectric point is 5.22, which may be located on mitochondria. Phylogenetic analysis showed that the peanut mitochondrial ACP protein was divided into two branches: AhmtACP1 and AhmtACP2 have relatively close relationship with AhmtACP3. Fluorescence quantitative PCR results showed that AhmtACP3 gene expression in flowers was significantly higher than other tissues, followed by cotyledons and roots. AhmtACP3 gene expression in seed development showed a downward trend.