文献已报道用异构酶、DNA分析及结合生物学研究等方法将旋毛虫属分为8种,即T_s、T_(ne)、T_(na)、T_(ps)、T_b及T_5、T_6、T_8。但以上鉴定技术需要培养许多活幼虫(历时40—50天),掌握特异的核苷酸序列的信息,并进行大量的数据分析,费时费力。为寻找一种简单、快速的鉴定方法。作者从保种的瑞士CD_1雌鼠及自然感染鼠的肌肉中取得40份旋毛虫分离物,在不同的PCR条件下用10或20 It has been reported in the literature that Trichinella species are classified into 8 species, namely T_s, T ne, T na, T ps, T_b and T_5, T_6, T_8. However, the above identification techniques need to cultivate many live larvae (which lasted 40-50 days), and it is time-consuming and laborious to grasp the information of specific nucleotide sequences and carry out a large amount of data analysis. To find a simple, rapid identification method. The authors obtained 40 copies of the trichinella isolates from the conserved swine CD 1 mice and the naturally infected mouse muscle using 10 or 20 different PCR conditions