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巯基乙酸盐(Thioglycollate)诱发的小鼠腹腔激活巨噬细胞显示了天然的抑制活性,即对Con A,PHA,PWM和LPS致分裂原诱导的T,B淋巴细胞的增殖能产生显著的抑制效应,同时也能抑制脾脏NK细胞对YAC-1肿瘤靶细胞的杀伤功能。但是这些巨噬细胞却同时具有明显的抗肿瘤的细胞静止效应。在体外用脂多糖(LPS,10微克/毫升)处理粘附性激活巨噬细胞明显地降低了对NK活性和T,B淋巴细胞增殖功能的抑制,然而这些免疫调变巨噬细胞仍然保持了显著的抗肿瘤的细胞静止效应。实验还表明,这类激活巨噬细胞的抑制功能不为前列腺素合成酶的抑制剂In-dom所阻断,从而提示了它们的免疫抑制效应似乎不是前列腺素介导的。本实验结果提示,体外用脂多糖处理后的免疫调变巨噬细胞是一类具有抗肿瘤功能和低免疫抑制性的巨噬细胞亚群,这为进一步研究它们的产生以及作用机理准备了条件。
Thioglycollate-induced mouse peritoneal activation of macrophages showed a natural inhibitory activity, that is, significant inhibition of proliferation of T, B lymphocytes induced by mitogen of Con A, PHA, PWM and LPS Effect, but also inhibit the killing function of spleen NK cells on YAC-1 tumor target cells. However, these macrophages at the same time have obvious anti-tumor cell quiescent effect. Treatment of adhesively-activated macrophages with lipopolysaccharide (LPS, 10 μg / ml) in vitro significantly reduced the inhibition of NK activity and T, B lymphocyte proliferation functions, however these immunomodulatory macrophages remained Significant anti-tumor cell quiescent effect. Experiments also show that the inhibitory function of these activated macrophages is not blocked by the prostaglandin synthase inhibitor In-dom, suggesting that their immunosuppressive effects do not appear to be prostaglandin-mediated. Our results suggest that immunosuppressed macrophages treated with lipopolysaccharide in vitro are a class of macrophage subsets with anti-tumor function and low immunosuppression, which are the conditions for further study of their production and mechanism of action .