论文部分内容阅读
目的:探讨吡那地尔(pinacidil)建立的短QT间期综合征模型致室性心律失常的机制,并观察缝隙连接激动剂抗心律失常肽(AAP10)对该模型电生理参数的影响。方法:利用pinacidil灌注家兔楔形心肌块建立短QT间期综合征模型。将20只新西兰长耳白兔随机分成pinacidil组和AAP10组,每组10只。pinacidil组灌流10μmol/L的pinacidil,AAP10组灌流AAP10500nmol/l和pinacidil10μmol/L的混合液,同步记录灌流前后内外膜动作电位和容积心电图,观察灌流前后QT间期,跨室壁离散度(TDR),程序性刺激观察心肌组织不应期和室性心律失常的诱发情况。结果:灌流pinacidil后,QT间期从(291±19)ms缩到(232±19)ms(P<0.05),TDR从(44±12)ms减少到(22±7)ms(P<0.05),而不应期从(164±8)ms减少到(112±14)ms(P<0.05),室性心律失常发生率从0/10增加至8/10(P<0.05)。AAP10组和pinacidil组的TDR、QT间期、不应期及室性心律失常的诱发率无显著差别。结论:TDR减小和不应期的缩短可能是pinacidil建立的短QT间期模型致室性心律失常的基础,AAP10对pinacidil诱导的短QT间期综合征模型电不稳定性无明显影响。
OBJECTIVE: To investigate the mechanism of ventricular arrhythmia induced by pinacidil in short QT syndrome and to observe the effect of gap junction agonist anti-arrhythmic peptide (AAP10) on electrophysiological parameters in this model. Methods: A short QT syndrome model was established by perfusion of pinchocardiogram with pinacidil. Twenty New Zealand white rabbits were randomly divided into pinacidil group and AAP10 group, 10 rats in each group. The perfusion of 10μmol / L pinacidil in the pinacidil group, AAP10500nmol / l and pinacidil10μmol / L perfusion in the AAP10 group were performed. The action potentials and volume electrocardiogram of the epicardium before and after perfusion were recorded simultaneously. The changes of QT interval, transmural dispersion (TDR) , Procedural stimulation observed myocardial tissue refractory period and induced ventricular arrhythmia. RESULTS: After infusion of pinacidil, the QT interval was reduced from (291 ± 19) ms to (232 ± 19) ms (P <0.05) and TDR decreased from (44 ± 12) ms to (22 ± 7) ms ), While the refractory period decreased from (164 ± 8) ms to (112 ± 14) ms (P <0.05). The incidence of ventricular arrhythmia increased from 0/10 to 8/10 (P <0.05). There was no significant difference in TDR, QT interval, refractory period and ventricular arrhythmia between AAP10 group and pinacidil group. Conclusions: Reduction of TDR and shortening of refractory period may be the basis of ventricular arrhythmia induced by pinacidil in short QT interval model. AAP10 has no obvious effect on the electrical instability induced by pinacidil in short QT syndrome.