论文部分内容阅读
本研究针对悬滴培养法在操作和应用上存在的问题和局限性，改用操作简便、适用范围广的非悬滴开放式培养法培养鸡胚背根节。将数个鸡胚背根节按一定间隔种植在内置生长基质盖玻片的35mm培养皿中，加人适量培养液，置于CO2。孵箱中进行培养。结果显示.从培养24h至60h各时期，培养皿中背根节生长状况均良好，神经突起明显增长，表明用非悬滴开放式培养法培养鸡胚背根节是可行且可靠的。
In this study, the hanging drop culture method in the operation and application of the existing problems and limitations, the use of simple, wide range of applications of non-hanging open-cell culture suspension culture of chicken dorsal root. Several chicken embryo dorsal root segments were planted at regular intervals in a 35 mm Petri dish with a built-in growth substrate coverslip and added with a suitable amount of culture medium and placed in CO2. Incubation in the incubator. The results showed that the growth of dorsal root ganglion was well and the number of neurite was obviously increased from 24h to 60h in culture, which indicated that it was feasible and reliable to culture chick embryo dorsal root ganglion with non-hanging drop open culture method.