论文部分内容阅读
目的研究木材、面粉加工场所鼠类自然感染肠螨情况。方法对捕获鼠类进行粪检螨阳性调查并对结肠组织进行病理分析;提取鼠类结肠组织总DNA,PCR检测粉螨细胞色素氧化酶I亚基因(COⅠ)。结果调查济宁市任城区木材、面粉加工场所以及社区鼠类孳生场所10处,共捕鼠19只。鉴定鼠体表检出粗脚粉螨、腐酪食螨、粉尘螨、乳果螨和谷跗线螨。解剖结肠做粪检,螨阳性者2例;村镇木材加工、仓库杂货店鼠类肠螨感染率显著高于城市社区(χ2=160.27,P<0.01),显示差异有统计学意义。PCR检测显示在380bp处出现阳性条带,与粪检阳性结果一致。鼠类阳性结肠组织经病理分析与肠螨病的典型特征相吻合。结论调查显示鼠类自然感染肠螨情况与特定的环境有关,粉螨的COⅠ检测方法有利于肠螨调查。
Objective To study the natural infection of gut mites by rodents in wood and flour processing sites. Methods The murine mouses were screened for positive stools and pathological analysis of colonic tissues was performed. The total DNA of murine colon tissues was extracted and the COⅠ gene of acaroid mite cytochromes was detected by PCR. Results Nineteen mice were harvested in 10 sites of lumber, flour processing sites and community rodents in Jining City. Identify the rat body surface detected aphids mites, mousse mites, dust mites, milk and fruit mites and tarsal line mites. Anatomical colon fecal examination, mites were positive in 2 cases; village wood processing, warehouse grocery store mouse intestinal intestinal mite infection was significantly higher than the urban community (χ2 = 160.27, P <0.01), showing a statistically significant difference. PCR detection showed a positive band at 380bp, consistent with the positive stool tests. Murine positive colon tissue by pathological analysis and the typical characteristics of intestinal mite disease coincide. Conclusion The investigation shows that the natural infection of gut mites is related to specific environment. The COⅠ detection method of acaroid mites is beneficial to the investigation of intestinal mites.